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KMID : 1149220100180010095
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Korean Journal of Oriental Medical Prescription
2010 Volume.18 No. 1 p.95 ~ p.103
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Simultaneous Determination of Seven Compounds in Samsoeum by HPLC-PDA
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Seo Chang-Seob
Kim Jung-Hoon
Huang Dae-Sun
Shin Hyeun-Kyoo
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Abstract
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Objectives:To develop and validate HPLC-PDA methods for simultaneous determination of seven constituents in Samsoeum(SSE).
Methods:Reverse-phase chromatography using a Gemini C18 column operating at , and photodiode array(PDA) detection at 254 and 280 nm, were used for quantification of the seven marker components of SSE. The mobile phase using a gradient flow consisted of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile with 1.0% (v/v) acetic acid.
Results:Calibration curves were acquired with >0.9997, and the relative standard deviation (RSD) values (%) for intra- and inter-day precision were less than 3.0%. The recovery rate of each compound was in the range of 100.07-112.65%, with an RSD less than 4.0%. The contents of seven compounds in SSE were 1.24-10.53 mg/g.
Conclusions:The established method will be helpful to improve quality control of SSE.
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KEYWORD
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HPLC-PDA, Samsoeum(SSE), Shensuyin, simultaneous determination
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